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tet-on plko sherbb3  (Addgene inc)


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    Structured Review

    Addgene inc tet-on plko sherbb3
    Tet On Plko Sherbb3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tet-on+plko+sherbb3/sherbb3/pmc03515079-56-38-46
    Average 90 stars, based on 1 article reviews
    tet-on plko sherbb3 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: MEK inhibition leads to PI3K/AKT activation by relieving a negative feedback on ERBB receptors
    Article Snippet: Transient transfections of CHO-KI cells were performed with Trans IT®-LT1 Transfection Reagent (MirusBio LLC) according to the manufacturer’s recommendations. .. Wild-type ERBB3 was co-transfected with an equal ratio of GFP or wild-type or mutant EGFR or HER2. shRNA, DNA Constructs and Lentiviral Production HCC827 cells were infected with shERBB3 as previously described ( 23 , 24 ) with tet-on PLKO shERBB3 or scramble shRNA knockdown vectors (Addgene) and selected in puromycin. shRNA hairpin sequences are provided in the Supplemental Materials and Methods . .. Human EGFR (wild-type and exon 19del) and HER2 cDNA coding regions were cloned into the pENTR/D-TOPO vector (Invitrogen) and mutants were constructed with Quick Change Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s instructions.

    shRNA:

    Article Title: MEK inhibition leads to PI3K/AKT activation by relieving a negative feedback on ERBB receptors
    Article Snippet: Transient transfections of CHO-KI cells were performed with Trans IT®-LT1 Transfection Reagent (MirusBio LLC) according to the manufacturer’s recommendations. .. Wild-type ERBB3 was co-transfected with an equal ratio of GFP or wild-type or mutant EGFR or HER2. shRNA, DNA Constructs and Lentiviral Production HCC827 cells were infected with shERBB3 as previously described ( 23 , 24 ) with tet-on PLKO shERBB3 or scramble shRNA knockdown vectors (Addgene) and selected in puromycin. shRNA hairpin sequences are provided in the Supplemental Materials and Methods . .. Human EGFR (wild-type and exon 19del) and HER2 cDNA coding regions were cloned into the pENTR/D-TOPO vector (Invitrogen) and mutants were constructed with Quick Change Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s instructions.

    Construct:

    Article Title: MEK inhibition leads to PI3K/AKT activation by relieving a negative feedback on ERBB receptors
    Article Snippet: Transient transfections of CHO-KI cells were performed with Trans IT®-LT1 Transfection Reagent (MirusBio LLC) according to the manufacturer’s recommendations. .. Wild-type ERBB3 was co-transfected with an equal ratio of GFP or wild-type or mutant EGFR or HER2. shRNA, DNA Constructs and Lentiviral Production HCC827 cells were infected with shERBB3 as previously described ( 23 , 24 ) with tet-on PLKO shERBB3 or scramble shRNA knockdown vectors (Addgene) and selected in puromycin. shRNA hairpin sequences are provided in the Supplemental Materials and Methods . .. Human EGFR (wild-type and exon 19del) and HER2 cDNA coding regions were cloned into the pENTR/D-TOPO vector (Invitrogen) and mutants were constructed with Quick Change Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s instructions.

    Infection:

    Article Title: MEK inhibition leads to PI3K/AKT activation by relieving a negative feedback on ERBB receptors
    Article Snippet: Transient transfections of CHO-KI cells were performed with Trans IT®-LT1 Transfection Reagent (MirusBio LLC) according to the manufacturer’s recommendations. .. Wild-type ERBB3 was co-transfected with an equal ratio of GFP or wild-type or mutant EGFR or HER2. shRNA, DNA Constructs and Lentiviral Production HCC827 cells were infected with shERBB3 as previously described ( 23 , 24 ) with tet-on PLKO shERBB3 or scramble shRNA knockdown vectors (Addgene) and selected in puromycin. shRNA hairpin sequences are provided in the Supplemental Materials and Methods . .. Human EGFR (wild-type and exon 19del) and HER2 cDNA coding regions were cloned into the pENTR/D-TOPO vector (Invitrogen) and mutants were constructed with Quick Change Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s instructions.

    Knockdown:

    Article Title: MEK inhibition leads to PI3K/AKT activation by relieving a negative feedback on ERBB receptors
    Article Snippet: Transient transfections of CHO-KI cells were performed with Trans IT®-LT1 Transfection Reagent (MirusBio LLC) according to the manufacturer’s recommendations. .. Wild-type ERBB3 was co-transfected with an equal ratio of GFP or wild-type or mutant EGFR or HER2. shRNA, DNA Constructs and Lentiviral Production HCC827 cells were infected with shERBB3 as previously described ( 23 , 24 ) with tet-on PLKO shERBB3 or scramble shRNA knockdown vectors (Addgene) and selected in puromycin. shRNA hairpin sequences are provided in the Supplemental Materials and Methods . .. Human EGFR (wild-type and exon 19del) and HER2 cDNA coding regions were cloned into the pENTR/D-TOPO vector (Invitrogen) and mutants were constructed with Quick Change Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s instructions.



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